Life science technology · Indiainfo@aceprobe.com
AceProbe — Probing Molecules For Life
NGS kits & reagents

Build around your sample.
Plan the sequencing workflow.

Discuss library-preparation kits, sequencing reagents and workflow consumables. Selection starts with your sample, protocol and platform compatibility.

Selected product specifications

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5 catalogue entries

Cleanup & size selection

DNA Size-Selection Beads

Catalogue reference: GNG316

Magnetic-bead chemistry for selecting DNA fragment sizes and cleaning up libraries without gel extraction.

Specifications & formats
DNA fragment range
100–1,000 bp
DNA type
Single- and double-stranded DNA
Selection approach
Adjust the bead-to-sample ratio
Pack formats
15 mL or 60 mL
Storage
2–8°C
Shelf life
12 months under stated storage conditions
Additional reagent
Freshly prepared 80% ethanol
Workflow
Manual or automated high-throughput processing
Catalogue / pack
GNG316-01 · 4995262 · 15 mL
Catalogue / pack
GNG316-02 · 4995261 · 60 mL

The cleanup removes residual dNTPs, salts and organic contaminants. Selection conditions depend on the desired fragment range; follow the supplied protocol.

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Enzymatic fragmentation

DNA Fragmentation Module

Catalogue reference: GNG305

An enzyme-based module for breaking double-stranded DNA into fragments ahead of NGS library construction.

Specifications & formats
DNA input
1 ng–1 µg
Target fragment range
200–500 bp
Size control
Adjust fragmentation reaction time
Fragment ends
Blunt-ended; 5′ phosphate and 3′ hydroxyl
Pack formats
24 or 96 reactions
Storage
−30 to −15°C; avoid repeated freeze–thaw cycles
Shelf life
12 months under stated storage conditions
Catalogue / pack
GNG305-01 · 4992356 · 24 reactions
Catalogue / pack
GNG305-02 · 4992357 · 96 reactions

Downstream library construction requires the appropriate end-processing, ligation, amplification and cleanup reagents. Confirm the complete workflow at enquiry.

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Library preparation

Fast DNA Library Preparation Kit

Catalogue reference: GNG102

A library-preparation workflow for short or previously fragmented double-stranded DNA.

Specifications & formats
Sequencing compatibility
Illumina workflows
DNA input
0.25 ng–1 µg
Starting material
Short DNA fragments or pre-sheared double-stranded DNA
Combined step
End repair and 3′ dA-tailing in one tube
Next step
Adapter ligation without an intervening cleanup
Workflow duration
Approximately 2.5 hours; protocol-dependent
Pack formats
24 or 96 reactions
Storage
−30 to −15°C; avoid repeated freeze–thaw cycles
Shelf life
12 months under stated storage conditions
Catalogue / pack
GNG102-01 · 4992261 · 24 reactions
Catalogue / pack
GNG102-02 · 4992262 · 96 reactions

Includes library-amplification chemistry. Compatible adapters and size-selection beads are additional workflow requirements; confirm their inclusion separately.

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Library preparation with fragmentation

Integrated DNA Library Preparation Kit

Catalogue reference: GNG101

A single-tube starting step combines DNA fragmentation, end repair and 3′ dA-tailing before adapter ligation.

Specifications & formats
Sequencing compatibility
Illumina workflows
DNA input
1 ng–1 µg
Combined step
Fragmentation, end repair and 3′ dA-tailing
Next step
Adapter ligation without an intervening cleanup
Workflow duration
Approximately 2.5 hours; protocol-dependent
Pack formats
24 or 96 reactions
Handling
Avoid repeated freeze–thaw cycles
Catalogue / pack
GNG101-01 · 4992259 · 24 reactions
Catalogue / pack
GNG101-02 · 4992260 · 96 reactions

Includes library-amplification chemistry. Compatible adapters and size-selection beads are additional workflow requirements. Confirm current storage instructions and the complete kit configuration at enquiry.

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Library indexing

Dual-Index Adapters

Catalogue reference: GNG216

Indexing adapters for distinguishing samples when DNA or RNA libraries are pooled for sequencing.

Specifications & formats
Sequencing compatibility
Illumina workflows
Index design
Two 8-base index sequences per adapter
Adapter selection
96 types across four groups of 24
Supplied concentration
15 µM
Pack formats
96-preparation group packs; 384-preparation combined pack
Included accessory
Adapter dilution buffer
Storage
−30 to −15°C
Shelf life
24 months under stated storage conditions
Handling
Keep thawed adapters on ice; avoid repeated freeze–thaw cycles
Catalogue / pack
GNG216-A · 4995263 · 96 preparations; group A
Catalogue / pack
GNG216-B · 4995264 · 96 preparations; group B
Catalogue / pack
GNG216-C · 4995265 · 96 preparations; group C
Catalogue / pack
GNG216-D · 4995266 · 96 preparations; group D
Catalogue / pack
GNG216 · 4995269 · 384 preparations; combined

Adapter input depends on library chemistry, starting DNA amount and fragment size. The number of preparation reactions is distinct from the number of unique adapter types.

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Entries include product groups and individual pack variants. This catalogue is not a statement of stock. Current product format, compatibility, documentation, availability and commercial terms are confirmed with the quotation. Research-use suitability and handling must be checked against current product documentation.

Selection considerations

Consider each stage of the workflow.

01

Library preparation

Review input material, sample quality, library method and indexing needs against the manufacturer’s current protocol.

02

Sequencing reagents

Check the reagent configuration, read length and compatibility with the platform and protocol used in your laboratory.

03

Workflow consumables

Identify the consumables, handling conditions and quality-control steps required by your chosen method.

Plan your requirement

Compatibility is part of the requirement.

Samples & inputsSample type, nucleic-acid input, quality and number of samples
Workflow stageLibrary preparation, sequencing or a specific supporting step
CompatibilityPlatform and model, protocol version, library format and read configuration
Supply detailsCatalogue number if known, kit size, quantity and storage requirements
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