The Hemo ID Blood Group Genotyping Panel (Hemo ID Panel) is designed for use with the MassARRAY® System for the identification of key polymorphisms in erythrocyte, platelet, and neutrophil antigens, starting with genomic DNA. With the Hemo ID Panel, you can:
- Interrogate 167 polymorphisms in 101 antigens (16 blood group systems) and 23 platelet and neutrophil antigens.
- Perform just the analyses of interest and minimize time and material wastage.
- Select any individual Hemo ID Module for focused studies or use the complete Hemo ID Panel for comprehensive analyses.
- Obtain predicted phenotypes for up to 3000 samples in 6-8 hours.
- Be confident in the accuracy and precision of your results generated with the MassARRAY System2,3 a genetic analysis platform referenced in more than 2,400 scientific publications.
- Analyze complex Rhesus genotypes and identify variants with assays for RHD zygosity, Del, weak and partial RHD alleles, and RHD-RHCE hybrid alleles.
- View and print your results with intuitive reports in multiple formats, which allow you to drill down to more detailed data and to select your favorite phenotype designations. (ISBT genotype and phenotype are used as default references when available.)
- Reports include nine RHD zygosity assay plots to support user assignment of zero, one, or two copies of RHD.
- Design and run your own additional genotyping assays for blood group variants of interest in your local ethnic population using the on-line Assay Design Suite Software.
- Start your assay designs with just the dbSNP rs numbers, or dbSNP data in FASTA format for the SNP positions of interest.
- The data generated from additional multiplex wells is aggregated and reported with your Hemo ID Panel assay results.
|Hemo ID Blood Group Genotyping Panel|
|Sample Type||Genomic DNA, isolated from whole blood or other tissue|
|Sample Amount||20 ng gDNA/multiplex assay well|
|Samples per 96W plate||1 – 96 samples (depends on the number of multiplex wells in the Module)|
|Samples per 384W plate||1 – 384 samples (depends on the number of multiplex wells in the Module)|
|No. of wells||1-10 wells (depends on Module)|
|Analytical Sensitivity||>95% genotype call rate in internal tests with 20 ng of gDNA per well|
Module Descriptions and Blood Group Systems Assayed
Predicted phenotypes assayed by the Hemo ID Blood Group Genotyping Panel. The Panel contains six Modules, which you can purchase individually or together depending upon your research needs. Click on the Module name in the first column to view the SNP positions interrogated with each assay.
|Module Name (No. of multiplex reactions)||Predicted Phenotypes Assayed|
|Hemo ID Blood Group Genotyping PanelTen multiplex wells||Includes all six Hemo ID Modules for a comprehensive analysis of 16 blood group systems and 23 platelet and neutrophil antigens.|
|Hemo ID Kell, Kidd, Duffy ModuleSingle multiplex well||Kell: K/k, Kmod (2 variants), Kpa/Kpb, Jsa/Jsb, KEL11/KEL17, Knull or K0 (7 variants)Kidd: Jka/Jkb, Jk(a–b–) or Jk0 (3 variants)
Duffy: Fya/Fyb, Fy(a−b−) or Fy0 (GATA), Fy(b+w) or Fyx+
|Hemo ID MNS ModuleSingle multiplex well||M/N, S/s, Mta, Vw, Hut, IVS5 or P2|
|Hemo ID Rare Blood Groups ModuleTwo multiplex wells||Kell: K/k, Kpa/Kpb, Jsa/Jsb, KEL11/KEL17Other rare blood groups: Lua/Lub, Lu8/Lu14, Aua/Aub, Dia/Dib, Wra/Wrb, Yta/Ytb, Coa/Cob, Kna/Knb, McCa/McCb, Sla/Vil, Doa/Dob, Hy, Joa, LWa/LWb, Sc1/Sc2, Cra, Tca/Tcb/Tcc, Ina/Inb, Vel+/Vel–, Vel+weak (See Ref. 1)|
|Hemo ID RHD-RHCE Broad ModuleThree multiplex wells||Rhesus status: DD vs. Dd vs. dd , CC vs. Cc vs. cc, EE vs. Ee vs. ee, RHCE vs. RHD exon screening [promoter, intron 1, exon 3, 4, 5, 6, 7 (2x), intron 7, exon 9 (2x), exon 10], RHc at RHCE*201A, RHCwRHD weak type: Type 41, Type 66
RHD category & D– C+: cat DIII 4, cat DIVa, cat DIVa type, cat III 4-8 or DCes type 1, cat IIIsub or DCes type 1, Dces type 1 & 2 @ RHD-CE*1006T
D–: RHD(W16X), RHD(Y401X), RHD psi, RHD psi IVS3-19 dupl 37, RHD(IVS3+2T>A) null
RHDel: RHD(delA147), RHD(IVS3+1G>A)el, RHD(X418L)el D– (CE): RHD-CE(2-9)-D+203C, RHD-CE(2-9)-D-2+268A
Other Hybrids: RHD-CE(1-9)-D, RHD-CE(2-9)-D or RHD-CE(3-9)-D, RHD-CE(2-7)-D or RHD-CE(3-7)-D, RHD-CE(4-7)-D, RHCE-Ce-D(6-10); RHD-D-CE(3)-D, RHD-D-CE(3-5)-D, RHD-D-CE(3-6)-D, RHD-D-CE(4)-D, RHD-D-CE(4-5)-D, RHD-D-CE(4-6)-D, RHD-D-CE(5)-D, RHD-D-CE(5-9)-D, RHD-D-CE(6-9)-D, RHD-D-CE(7)-D, RHD-D-CE(7-9)-D, RHD-D-CE(1048C, 8-9)-D; RHCE-D(1-3)-CE, RHCE-ce-D(4-9)-ce, RHCE-Ce-D(4)-ce, RHCE-Ce-D(4)-ce + 455A, RHCE-CE-D(5)-CE, RHCE-ce-D(5)-ce
|Hemo ID RHD Variant Module Two multiplex wells||RHD weak type: Type 1, Type 1.1, Type 2, Type 3, Type 4.0, Type 4.1, Type 4.2 or DAR1, Type 4.3, Type 5, Type 11, Type 15, Type 17, Type 34, Type 47RHD category: cat DIII 6, cat DIII 7, cat DV 5, cat DVI1-DVI4, cat DVII
Other partial RHD: DAU1-DAU7, DFL, DFR1-DFR4, DHK, DNB, DOL1 or DAK, DOL3, DVL-2
Normal RHD: DAU0
RHCE variants: RHCE ce(G385A), RHCE CeMA
|Hemo ID HPA/HNA Module Single multiplex well
||HNA: HNA-1a/b/ab/ac/bc /c, HNA-3a/b, HNA-4a/bw, HNA-5a/bwHPA ( See Ref. 4 ): HPA-1a/b, HPA-2a/b, HPA-3a/b, HPA-4a/b, HPA-5a/b, HPA-6a/bw, HPA-15a/b|